IF-F, IF-IC
H M
Endogenous
Rabbit IgG
#Q15672
7291
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro24 of human TWIST1 protein.
Background
TWIST1 is a basic helix-loop-helix (b-HLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the b-HLH domain of the TWIST1 gene have been associated with Saethre-Chotzen syndrome, an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in EMT (epithelial-mesenchymal transition) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to Taxol and microtubule regulating drugs in tumors (8).
- Thisse, B. et al. (1987) Nucleic Acids Res. 15, 3439-53.
- Lee, M.S. et al. (1999) J. Cell Biochem. 75, 566-77.
- Chen, Z.F. and Behringer, R.R. (1995) Genes Dev. 9, 686-99.
- Howard, T.D. et al. (1997) Nat. Genet. 15, 36-41.
- Connerney, J. et al. (2006) Dev. Dyn. 235, 1345-57.
- Watanabe, O. et al. (2004) Anticancer Res. 24, 3851-6.
- Yang, J. et al. (2004) Cell 117, 927-39.
- Wang, X. et al. (2004) Oncogene 23, 474-82.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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