WB, IF-IC, FC-FP
H
Endogenous
25-40
Rabbit IgG
#Q15661
7177
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Tryptase protein.
Background
Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells (1). Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent) (2). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin (3-5). Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides (6), calcitonin gene-related peptides (7), fibronectins (8), fibrinogens (3), and kininogens (9). Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts (10-13).
- Vanderslice, P. et al. (1990) Proc Natl Acad Sci U S A 87, 3811-5.
- Krishnaswamy, G. et al. (2001) Front Biosci 6, D1109-27.
- Fukuoka, Y. and Schwartz, L.B. (2007) Int Immunopharmacol 7, 1900-8.
- Ren, S. et al. (1998) J Immunol 160, 4561-9.
- Sakai, K. et al. (1996) J Clin Invest 97, 988-95.
- Tam, E.K. and Caughey, G.H. (1990) Am J Respir Cell Mol Biol 3, 27-32.
- Walls, A.F. et al. (1992) Biochem Pharmacol 43, 1243-8.
- Lohi, J. et al. (1992) J Cell Biochem 50, 337-49.
- Walls, A.F. et al. (1992) Biochem Soc Trans 20, 260S.
- Cairns, J.A. and Walls, A.F. (1996) J Immunol 156, 275-83.
- Ruoss, S.J. et al. (1991) J Clin Invest 88, 493-9.
- Brown, J.K. et al. (1995) Am J Respir Cell Mol Biol 13, 227-36.
- Gruber, B.L. et al. (1997) J Immunol 158, 2310-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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