TRIP-4/ASC-1 Antibody (#2333) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

SOURCE:

Rabbit

UniProt ID:

#Q15650

Entrez-Gene Id:

9325

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TRIP-4/ASC-1 Antibody detects endogenous levels of total TRIP-4/ASC-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding glycine 109 of human TRIP-4/ASC-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TRIP-4 (thyroid hormone receptor interactor 4)/ASC-1 (activating signal cointegrator 1) is a widely expressed transcriptional coactivator of nuclear receptors (1-3). The protein contains a zinc finger motif that serves as a binding site for basal transcription factors, TATA-binding protein (TBP) and TFIIA as well as SRC-1, CBP/p300 and nuclear receptors. Subcellular localization of TRIP-4/ASC-1 may depend on the cellular context. While it is normally expressed in the nucleus, TRIP-4/ASC-1 was observed in the cytoplasm under serum starvation but was retained in the nucleus when expressed with CBP or SRC-1 (2). TRIP-4/ASC-1 was also found to complex with AP-1, SRF and NF-κB and may play an important role in transactivation by these proteins as well as mediate their cross-talk with nuclear receptors (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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