WB, IP
H
Endogenous
33
Rabbit IgG
#Q9NSU2
11277
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro298 of human TREX1 protein.
Background
TREX1 is a broadly expressed 3’ to 5’ exonuclease that acts on single-stranded DNA (ssDNA) to negatively regulate the interferon-stimulatory DNA (ISD) response (1-4). In humans, there are three TREX1 isoforms generated through alternative splicing with predicted molecular weights of 32, 33, and 39 kDa (2). The transcript for the 33 kDa isoform is the most abundant (2). Mice deficient in TREX1 accumulate intracellular ssDNA, which triggers the ISD response and eventually lethal autoimmunity (3,4). Mutations in TREX1 are associated with autoimmune diseases including Aicardi-Goutieres syndrome and systemic lupus erythematosus (5,6). In addition, TREX1 prevents the cell-intrinsic innate immune response to human immunodeficiency virus (HIV) by digesting excess HIV DNA that would normally trigger induction of type I interferon (7).
- Mazur, D.J. and Perrino, F.W. (1999) J Biol Chem 274, 19655-60.
- Mazur, D.J. and Perrino, F.W. (2001) J Biol Chem 276, 14718-27.
- Yang, Y.G. et al. (2007) Cell 131, 873-86.
- Stetson, D.B. et al. (2008) Cell 134, 587-98.
- Crow, Y.J. et al. (2006) Nat Genet 38, 917-20.
- Lee-Kirsch, M.A. et al. (2007) Nat Genet 39, 1065-7.
- Yan, N. et al. (2010) Nat Immunol 11, 1005-13.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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