| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| eIF4A (C32B4) Rabbit mAb | 2013 | 20 µl | 48 kDa | Rabbit IgG |
| eIF4A1 Antibody | 2490 | 20 µl | 48 kDa | Rabbit |
| Phospho-eIF4B (Ser422) Antibody | 3591 | 20 µl | 80 kDa | Rabbit |
| eIF4B Antibody | 3592 | 20 µl | 80 kDa | Rabbit |
| Phospho-eIF4E (Ser209) Antibody | 9741 | 20 µl | 25 kDa | Rabbit |
| eIF4E (C46H6) Rabbit mAb | 2067 | 20 µl | 25 kDa | Rabbit IgG |
| Phospho-eIF4G (Ser1108) Antibody | 2441 | 20 µl | 220 kDa | Rabbit |
| eIF4G (C45A4) Rabbit mAb | 2469 | 20 µl | 220 kDa | Rabbit |
| eIF4H (D85F2) XP® Rabbit mAb | 3469 | 20 µl | 25, 27 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Translation Initiation Complex Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.
Storage
Background
A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7).
- Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608.
- Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-94.
- Sonenberg, N. et al. (1978) Proc Natl Acad Sci U S A 75, 4843-7.
- Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
- Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87.
Background References
Trademarks and Patents
限制使用
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