WB, IP
H M R Mk
Endogenous
67
Rabbit IgG
#P49116
7182
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile10 of human TR4/NR2C2 protein.
Background
Testicular receptor 4 (TR4), also called TAK1 or NR2C2, is an orphan receptor in a distinct subclass of the steroid hormone nuclear receptor superfamily along with TR2 (1,2). TR4 forms heterodimers with TR2 and binds to DNA elements containing direct repeats (DRs) (3). TR4 plays a role in various processes, including neurogenesis, spermatogenesis, RNA metabolism, and protein translation (4-6). TR4 can interact with other hormone receptors such as AR and ER to influence protein-protein binding and target gene inactivation (7,8). High expression of TR4 correlates with prostate cancer metastasis and invasion through downstream targets such as CCL2 and Ezh2 (9-10). Expression of TR4 in other cancers such as NSCLC and testicular germ cell tumors has also been associated with poor prognosis (11-12).
- Chang, C. et al. (1994) Proc Natl Acad Sci U S A 91, 6040-4.
- Law, S.W. et al. (1994) Gene Expr 4, 77-84.
- Lee, C.H. et al. (1998) J Biol Chem 273, 25209-15.
- Young, W.J. et al. (1998) J Biol Chem 273, 20877-85.
- Mu, X. et al. (2004) Mol Cell Biol 24, 5887-99.
- O'Geen, H. et al. (2010) BMC Genomics 11, 689.
- Shyr, C.R. et al. (2002) J Biol Chem 277, 14622-8.
- Lee, Y.F. et al. (1999) Proc Natl Acad Sci U S A 96, 14724-9.
- Ding, X. et al. (2015) Int J Cancer 136, 955-64.
- Zhu, J. et al. (2015) Mol Cancer Ther 14, 1445-53.
- Zhang, L. et al. (2015) Lung Cancer 89, 320-8.
- Chen, Y. et al. (2018) Cancer Sci 109, 384-394.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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