WB
H Mk
Endogenous
100
Rabbit
#Q9ULW0
22974
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).
- Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
- Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
- Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
- Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
- Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
- Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
- Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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