Transfected Only
60, 62
Rabbit
#P41279
1326
Product Information
Storage
Specificity / Sensitivity
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of rat Tpl2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Tpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine/threonine kinase expressed primarily in hematopoietic tissues, lung and liver (1). Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5 (2,3). Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1 (4,5). Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription (6). Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3 (7).
- Patriotis, C. et al. (1993) Proc Natl Acad Sci U S A 90, 2251-5.
- Salmeron, A. et al. (1996) EMBO J 15, 817-26.
- Chiariello, M. et al. (2000) Mol Cell Biol 20, 1747-58.
- Lin, X. et al. (1999) Immunity 10, 271-80.
- Belich, M.P. et al. (1999) Nature 397, 363-8.
- Kane, L.P. et al. (2002) Mol Cell Biol 22, 5962-74.
- Patriotis, C. et al. (2001) J Cell Physiol 187, 176-87.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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