WB, IF-F, IF-IC, FC-FP
H M R
Endogenous
60-80
Rabbit IgG
#Q96NM4, #O94900
84969, 9760
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln435 of human Tox protein.
Background
Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).
Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3+ T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14).
- O'Flaherty, E. and Kaye, J. (2003) BMC Genomics 4, 13.
- Aliahmad, P. et al. (2012) Curr Opin Immunol 24, 173-7.
- Wilkinson, B. et al. (2002) Nat Immunol 3, 272-80.
- Aliahmad, P. et al. (2004) J Exp Med 199, 1089-99.
- Chi, T.H. et al. (2002) Nature 418, 195-9.
- Aliahmad, P. and Kaye, J. (2008) J Exp Med 205, 245-56.
- Aliahmad, P. et al. (2010) Nat Immunol 11, 945-52.
- Yun, S. et al. (2011) Immunol Lett 136, 29-36.
- Page, N. et al. (2018) Immunity 48, 937-950.e8.
- Alfei, F. et al. (2019) Nature 571, 265-9.
- Yao, C. et al. (2019) Nat Immunol 20, 890-901.
- Wang, X. et al. (2019) J Hepatol pii: S0168-8278(19)30301-0. doi: 10.1016/j.jhep.2019.05.015.
- Morimura, S. et al. (2014) Arch Dermatol Res 306, 843-9.
- Huang, Y. et al. (2014) Oncotarget 5, 4418-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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