TOM70 (E7E1M) Rabbit mAb (#65619) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IF-F, IF-IC

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O94826

Entrez-Gene Id:

9868

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Frozen)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:3200 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TOM70 (E7E1M) Rabbit mAb recognizes endogenous levels of total TOM70 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala189 of human TOM70 protein.

Background

Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome, while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1).

TOM70 serves as a docking partner for cytosolic chaperone proteins and thus participates in the uptake of newly synthesized chaperone-bound proteins during mitochondrial biogenesis (2). In addition, TOM70 may play a role in Parkinson's disease since it is involved in recruiting PINK1 and Parkin to mitochondria. PINK1 and Parkin are activated after mitochondrial membrane depolarization, thus identifying uncoupled and damaged mitochondria (3). In addition, the protein encoded by Orf9b in the genome of SARS-CoV-2 binds to TOM70, leading to suppression of interferon responses (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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