WB, IP
H
Endogenous
22
Rabbit
#Q5BJD5
440026
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM41B protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
TMEM41B (also known as Stasimon) was discovered as a target of splicing dysfunction induced by loss of spinal motor neuron (SMN) protein, a feature of spinal muscular atrophy (SMA), resulting in motor circuit defects (1). Loss of TMEM41B in mice results in embryonic lethality (2). TMEM41B is a transmembrane protein that resides at the endoplasmic reticulum (ER) and is found at contact sites between the ER and mitochondria (2). Independent CRISPR screens identified TMEM41B as a novel regulator of autophagy, being structurally and functionally related to VMP1 (3-5). Loss of TMEM41B inhibited autophagosome formation and autophagic flux (3-5). Furthermore, depletion of TMEM41B led to an accumulation of lipid droplets, indicating a role in lipid mobilization and utilization of fatty acids (3,4). TMEM41B also appeared in screens, identifying it as an essential host protein for infection of flaviviruses and coronaviruses, including SARS-CoV-2 (6,7).
- Lotti, F. et al. (2012) Cell 151, 440-54.
- Van Alstyne, M. et al. (2018) Biochem Biophys Res Commun 506, 463-470.
- Moretti, F. et al. (2018) EMBO Rep19, e45889. doi: 10.15252/embr.201845889.
- Morita, K. et al. (2018) J Cell Biol 217, 3817-3828.
- Shoemaker, C.J. et al. (2019) PLoS Biol 17, e2007044.
- Hoffmann, H.H. et al. (2020) bioRxiv, 2020.10.09.334128. doi: 10.1101/2020.10.09.334128.
- Schneider, W.M. et al. (2020) bioRxiv, 2020.10.07.326462. doi: 10.1101/2020.10.07.326462.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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