WB, IHC-P
H Mk
Endogenous
26
Rabbit IgG
#P01033
7076
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
Storage
For a carrier free (BSA and azide free) version of this product see product #76074.
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala134 of human TIMP1 protein.
Background
TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases (MMPs) that includes TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by directly binding to their catalytic zinc cofactor and resultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include TIMP1 with CD63, TIMP2 with α3β1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby's fundus dystrophy (8,9).
- Nagase, H. et al. (2006) Cardiovasc Res 69, 562-73.
- Visse, R. and Nagase, H. (2003) Circ Res 92, 827-39.
- Stetler-Stevenson, W.G. (2008) Sci Signal 1, re6.
- Noel, A. et al. (2004) J Clin Pathol 57, 577-84.
- Hojilla, C.V. et al. (2003) Br J Cancer 89, 1817-21.
- Gill, S.E. and Parks, W.C. (2008) Int J Biochem Cell Biol 40, 1334-47.
- Raffetto, J.D. and Khalil, R.A. (2008) Biochem Pharmacol 75, 346-59.
- Weber, B.H. et al. (1994) Nat Genet 8, 352-6.
- Yeow, K.M. et al. (2002) Matrix Biol 21, 75-88.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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