Name: TIM-3 (D3M9R) Feline Chimeric Monoclonal Antibody
Price: 161.00 USD
Availability: InStock

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IHC

Product Specifications

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Feline chimeric IgG1

Application Key:

IHC-Immunohistochemistry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as TIM-3 (D3M9R) Rabbit Monoclonal Antibody #83882.

Product Usage Information

Application	Dilution
Immunohistochemistry (Paraffin)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

TIM-3 (D3M9R) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total TIM-3 protein.

Species Reactivity:

Mouse

Source / Purification

This recombinant chimeric antibody is engineered from TIM-3 (D3M9R) Rabbit Monoclonal Antibody #83882 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, TIM-3 (D3M9R) Rabbit Monoclonal Antibody #83882, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro137 of mouse TIM-3 protein.

Background

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).

Alternate Names

CD366; HAVcr-2; Havcr2; HAVR2; hepatitis A virus cellular receptor 2; Hepatitis A virus cellular receptor 2 homolog; MGC124149; MGC124150; OTTMUSP00000005654; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim; TIM-; TIM-3; Tim3; Timd; TIMD-3; Timd3

Database Links

UniProt ID: Q8VIM0

Entrez-Gene Id: 171285

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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TIM-3 (D3M9R) Feline Chimeric Monoclonal Antibody #73028