WB, IHC-P
H
Endogenous
50, 90-140
Rabbit IgG
#Q96D42
26762
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #71010.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val24 of human TIM-1 protein.
Background
T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus (1). TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production (2-5). Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1 (6). TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease (7). NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma (8). TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production (9). Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease (10,11). In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma (12,13). Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury (14).
- Kaplan, G. et al. (1996) EMBO J 15, 4282-96.
- Umetsu, S.E. et al. (2005) Nat Immunol 6, 447-54.
- de Souza, A.J. et al. (2005) Proc Natl Acad Sci U S A 102, 17113-8.
- Binné, L.L. et al. (2007) J Immunol 178, 4342-50.
- de Souza, A.J. et al. (2008) J Immunol 180, 6518-26.
- Meyers, J.H. et al. (2005) Nat Immunol 6, 455-64.
- Angiari, S. et al. (2014) Immunity 40, 542-53.
- Kim, H.S. et al. (2010) J Immunol 184, 4095-106.
- Lee, H.H. et al. (2010) J Immunol 185, 5225-35.
- Xiao, S. et al. (2012) Proc Natl Acad Sci U S A 109, 12105-10.
- Xiao, S. et al. (2015) J Immunol 194, 1602-8.
- McIntire, J.J. et al. (2001) Nat Immunol 2, 1109-16.
- Khademi, M. et al. (2004) J Immunol 172, 7169-76.
- Ichimura, T. et al. (1998) J Biol Chem 273, 4135-42.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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