Tid-1 (RS13) Mouse mAb

Product Usage Information

Storage

Specificity / Sensitivity

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Tid-1 protein. Antibody is supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 mg/ml BSA and 50% glycerol.

Background

Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l (2) tid and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins (1). These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1) (2,3). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP (3).