ThPOK (D9V5T) Rabbit mAb (#13205) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O15156

Entrez-Gene Id:

51043

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

ThPOK (D9V5T) Rabbit mAb recognizes endogenous levels of total ThPOK protein. This antibody cross-reacts with a 40 kDa protein of unknown origin.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human ThPOK protein.

Background

The transcription factor Th-inducing POZ/Krüppel-like factor (ThPOK, ZBTB7B, cKROX, ZFP67) is a transcriptional repressor belonging to the POK/ZBTB family of lymphoid cell development regulators (1). ThPOK is best known as a signature CD4+ T_h cell transcription factor that is upregulated during the differentiation of CD4+ T_h but not CD8+ cytotoxic T cells (1). Expression of ThPOK in developing T cells represses expression of CD8 and cytotoxic T cell effector genes, and indirectly promotes expression of CD4 by antagonizing RUNX-mediated CD4 repression (2-4). ThPOK expression has also been observed in NKT cells and γδ T cells (5,6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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