WB
H M
Endogenous
48
Rabbit
#O15533
6892
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Tapasin protein. Antibodies are purified by peptide affinity chromatography.
Background
TAP-associated glycoprotein (Tapasin/TAPBP) is a type-I transmembrane ER glycoprotein encoded by an MHC-linked gene. Tapasin plays a critical role in the MHC class I-restricted antigen processing and presentation pathway. Research studies have demonstrated that Tapasin is an IFNγ-inducible component of the TAP-1/2 peptide transporter complex and, within the ER lumen, facilitates the optimization of the peptide repertoire that is loaded onto MHC class I molecules (1-5). Downregulation of Tapasin and MHC class I expression has been observed in human tumors and serves as a prominent mechanism of tumor immune escape (6-9).
- Li, S. et al. (1997) Proc Natl Acad Sci U S A 94, 8708-13.
- Praveen, P.V. et al. (2010) Eur J Immunol 40, 214-24.
- Ortmann, B. et al. (1997) Science 277, 1306-9.
- Williams, A.P. et al. (2002) Immunity 16, 509-20.
- Garbi, N. et al. (2003) Eur J Immunol 33, 264-73.
- Ogino, T. et al. (2003) Clin Cancer Res 9, 4043-51.
- Cabrera, C.M. et al. (2005) Int J Cancer 113, 611-8.
- Sokol, L. et al. (2015) J Transl Med 13, 279.
- Jiang, Q. et al. (2010) Tumour Biol 31, 451-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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