WB, IP, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq, C&R
H
Endogenous
58-68
Rabbit IgG
#Q9UL17
30009
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #27112.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein.
Background
The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11).
- Wilkinson, D.G. et al. (1990) Nature 343, 657-9.
- Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19.
- Showell, C. et al. (2004) Dev Dyn 229, 201-18.
- Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70.
- Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60.
- Baldini, A. (2004) Curr Opin Cardiol 19, 201-4.
- Abrahams, A. et al. (2010) IUBMB Life 62, 92-102.
- Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9, 109-18.
- Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.
- Ho, I.C. and Glimcher, L.H. (2002) Cell 109 Suppl, S109-20.
- Peng, S.L. (2006) Cell Mol Immunol 3, 87-95.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq C&R: CUT&RUN
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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