Revision 3

#12922Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, FC-FP

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

180-250, 70

Source/Isotype:

Rabbit IgG

UniProt ID:

#P18827

Entrez-Gene Id:

6382

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Syndecan 1 (D4Y7H) Rabbit mAb recognizes endogenous levels of multimeric form of syndecan 1 protein. This antibody cross-reacts with proteins of unknown origin between 46-60 kDa in some cell lines.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala294 of human syndecan 1 protein.

Background

Syndecans are a family of type 1 transmembrane heparan sulfate proteoglycans comprising four members in mammals (SDC1-4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins, including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4).

Syndecan 1 (SDC1) is a specific marker for plasmacytic differentiation in hematologic disorders (5-7). This cell surface proteoglycan is also expressed in normal epithelial cells and tissues as well as various types of cancer tissues (8-11). The extracellular shed form of syndecan 1 remains soluble or accumulates in the extracellular matrix where it binds growth factors, cytokines and other extracellular matrix proteins (12,13). This binding activates signaling of bound growth factors or cytokines, which results in enhanced tumor growth, dissemination, angiogenesis, and osteolysis (14-17). As a result, the level of syndecan1 protein and its shed form may serve as prognostic factors for a list of malignancies (6,18,19). Syndecan 1 has recently been found to be a critical mediator of macropinocytosis in pancreatic cancer (20).

  1. Couchman, J.R. (2003) Nat Rev Mol Cell Biol 4, 926-37.
  2. Multhaupt, H.A. et al. (2009) J Physiol Pharmacol 60 Suppl 4, 31-8.
  3. Zimmermann, P. and David, G. (1999) FASEB J 13 Suppl, S91-S100.
  4. Manon-Jensen, T. et al. (2010) FEBS J 277, 3876-89.
  5. Chilosi, M. et al. (1999) Mod Pathol 12, 1101-6.
  6. Seidel, C. et al. (2000) Blood 95, 388-92.
  7. O'Connell, F.P. et al. (2004) Am J Clin Pathol 121, 254-63.
  8. Inki, P. and Jalkanen, M. (1996) Ann Med 28, 63-7.
  9. Matsumoto, A. et al. (1997) Int J Cancer 74, 482-91.
  10. Conejo, J.R. et al. (2000) Int J Cancer 88, 12-20.
  11. Zellweger, T. et al. (2003) Prostate 55, 20-9.
  12. Bayer-Garner, I.B. et al. (2001) Mod Pathol 14, 1052-8.
  13. Ramani, V.C. et al. (2013) FEBS J 280, 2294-306.
  14. Derksen, P.W. et al. (2002) Blood 99, 1405-10.
  15. You, W.K. and McDonald, D.M. (2008) BMB Rep 41, 833-9.
  16. Ramani, V.C. et al. (2011) J Biol Chem 286, 6490-9.
  17. Aragão, A.Z. et al. (2012) PLoS One 7, e43521.
  18. Joensuu, H. et al. (2002) Cancer Res 62, 5210-7.
  19. Lee, S.H. et al. (2013) Int J Clin Oncol (Epub ahead of print).
  20. Yao, W. et al. (2019) Nature 568, 410-414.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
仅供研究使用。不得用于诊断流程。