WB
H M R Mk
Endogenous
40
Rabbit
#O75494
10772
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His167 of human SRSF10 protein. Antibodies are purified by peptide affinity chromatography.
Background
Serine/arginine-rich splicing factor 10 (SRSF10), also known as TASR or SRp38, was initially described as a splicing factor associated with the FUS oncoprotein (1,2). Upon dephosphorylation, SRSF10 represses splicing to regulate numerous functions, including cell cycle, heat shock response, neurogenesis, and adipocyte differentiation (3-6). SRSF10 is affected by adenosine methylation, as it has been shown to be excluded from nuclear speckles by m6A-binding protein YTHDC1 (7). SRSF10 splicing repression has been implicated in colon and cervical cancer (8,9).
- Yang, L. et al. (1998) J Biol Chem 273, 27761-4.
- Yang, L. et al. (2000) Mol Cell Biol 20, 3345-54.
- Shin, C. and Manley, J.L. (2002) Cell 111, 407-17.
- Shin, C. et al. (2004) Nature 427, 553-8.
- Liu, K.J. and Harland, R.M. (2005) Development 132, 1511-23.
- Li, H. et al. (2014) Mol Cell Biol 34, 2198-207.
- Xiao, W. et al. (2016) Mol Cell 61, 507-19.
- Zhou, X. et al. (2014) Nat Commun 5, 4581.
- Liu, F. et al. (2018) Oncogene 37, 2394-409.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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