WB, IP
H
Endogenous
50
Rabbit IgG
#Q7Z699
161742
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln281 of human Spred1 protein.
Background
Spred1 belongs to the Spred family functioning as sprouty-related suppressor of Ras signaling down stream of growth receptor signaling (1,2). The protein has a N-terminal EVH1 (Ena/VASP homology-1) domain, followed by a KBD (c-Kit- binding domain) and a C-terminal SPR (Sprouty) domain. The C-terminal SPR domain functions to translocate Spred1 to the membrane following growth factor stimulation (1,3). The N-terminal EVH1 domain interacts with neurofibromin to bring the later to the membrane proximal signaling complex for down regulation of Ras signaling (4,5). Mutations of Spred1 are the causes of Legius Syndrome (6,7). Spred1 expression inhibits tumor cell motility and metastasis, and down regulation has been found in hepatocarcinoma and myeloblastic leukemia (8-10).
- Wakioka, T. et al. (2001) Nature 412, 647-51.
- Kato, R. et al. (2003) Biochem Biophys Res Commun 302, 767-72.
- King, J.A. et al. (2005) Biochem J 388, 445-54.
- Stowe, I.B. et al. (2012) Genes Dev 26, 1421-6.
- Hirata, Y. et al. (2015) J Biol Chem , .
- Brems, H. et al. (2007) Nat Genet 39, 1120-6.
- Brems, H. and Legius, E. (2013) Keio J Med 62, 107-12.
- Miyoshi, K. et al. (2004) Oncogene 23, 5567-76.
- Yoshida, T. et al. (2006) Oncogene 25, 6056-66.
- Pasmant, E. et al. (2015) Oncogene 34, 631-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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