WB, IP
H M R Mk
Endogenous
29
Rabbit IgG
#Q9Y657
10927
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His33 of human SPIN1 protein.
Background
SPIN1, also known as Spindlin1, was first identified as a spindle binding protein during meiosis in mice (1). SPIN1 contains tandem tudor-like domains, which can recognize methylation of H3K4, H4K20, and H4R23 (2-4). SPIN1 localizes to rRNA genes to help drive their expression, and has also been shown to promote cellular proliferation through binding TCF4 to activate wnt signaling (2,5). This transcriptional activity is opposed by the SPIN1 docking protein SPINDOC, a repressor which causes SPIN1 to dissociate from chromatin (6). SPIN1 has shown to be overexpressed in ovarian cancers, and in other cell types SPIN1 overexpression causes cell cycle arrest and chromosome instability (7,8). SPIN1 has also been identified as a potential target in liposarcomas, where it contributes to RET signaling by controlling the expression of the activator GDNF (9).
- Oh, B. et al. (1997) Development 124, 493-503.
- Wang, W. et al. (2011) EMBO Rep 12, 1160-6.
- Zhao, Q. et al. (2007) J Biol Chem 282, 647-56.
- Wang, C. et al. (2018) FEBS Lett 592, 4098-110.
- Wang, J.X. et al. (2012) Mol Cancer Res 10, 326-35.
- Bae, N. et al. (2017) J Biol Chem 292, 20808-17.
- Yue, W. et al. (2004) Ai Zheng 23, 141-5.
- Yuan, H. et al. (2008) Gene 410, 67-74.
- Franz, H. et al. (2015) Oncotarget 6, 4773-89.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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