Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit mAb (#63335) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, IF-IC

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

40-50

Source/Isotype:

Rabbit IgG

UniProt ID:

#P21453

Entrez-Gene Id:

1901

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:1600 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit mAb recognizes endogenous levels of total sphingosine-1-phosphate receptor 1 protein. Nonspecific nuclear background may be observed by immunofluorescence in some cell lines, especially with higher than recommended concentrations of antibody.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly366 of human sphingosine-1-phosphate receptor 1 protein.

Background

Sphingosine-1-phosphate receptor 1 (S1PR1, EDG1) is a G protein-coupled receptor (GPCR), one of five members (S1PR1-5) belonging to the sphingosine-1-phosphate receptor (S1PR) family (1,2). This family of GPCRs has been shown to exhibit broad and often overlapping expression patterns across a wide array of tissues. The cognate ligand for S1PRs is sphingosine-1-phosphate (S1P), a phosphorylated lipid signaling molecule derived from sphingosine. S1P/S1PR binding can trigger the activation and/or inhibition of various downstream signaling pathways, depending on context. Pathways identified downstream of S1P/S1PR include PI3K/Akt, PLC, Ras/MAPK and PKA (3,4). S1P/S1PR1 signaling pathways play important roles in many basic cellular functions during normal development, including chemotaxis, vascular integrity, and immune cell egress, but can also contribute to a variety of pathological conditions, including atherogenesis, multiple sclerosis, and tumorigenesis (5-9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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