WB
H M R
Endogenous
28, 30
Rabbit IgG
#P08294
6649
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human SOD3 protein.
Background
Superoxide dismutase 3 (SOD3, EC-SOD) is a copper-containing antioxidant enzyme that plays a critical role in redox homeostasis in extracellular spaces (1). SOD3 localizes to the extracellular matrix through its C-terminal heparin binding domain, which interacts with proteoglycan on the cell surface (2). The enzyme catalyzes the dismutation of the superoxide radical (O2-) to hydrogen peroxide in the extracellular space, prevents oxidative fragmentation damage of the extracellular matrix under stress conditions (3), and protects against oxidant-mediated endothelial dysfunction and tissue inflammation (4,5). SOD3 antioxidative activity preserves the bioavailability of nitric oxide (NO), providing permissive signals through the normalization of tumor vasculature, leading to enhanced tumor infiltration by effector T cells (6,7). Downregulation of SOD3 provides a selective advantage for multiple cancer types (8). A SOD3 knockout animal model displays resistance to chemotherapy response, while overexpression of SOD3 increases the tumor response to chemotherapy (9).
- Fukai, T. and Ushio-Fukai, M. (2011) Antioxid Redox Signal 15, 1583-606.
- Sah, S.K. et al. (2020) Cell Biosci 10, 22.
- Yao, H. et al. (2010) Proc Natl Acad Sci USA 107, 15571-6.
- Foresman, E.L. and Miller, F.J. (2013) Redox Biol 1, 292-6.
- Nguyen, N.H. et al. (2020) J Mol Med (Berl) 98, 59-69.
- Martínez-Rey, D. et al. (2020) Front Immunol 11, 579552.
- Carmona-Rodríguez, L. et al. (2020) Oncoimmunology 9, 1794163.
- Griess, B. et al. (2017) Free Radic Biol Med 112, 464-479.
- Mira, E. et al. (2018) Nat Commun 9, 575.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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