WB, IP
H M R Mk
Endogenous
29
Rabbit IgG
#O95863
6615
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human Snail protein. The epitope has been mapped to residues surrounding Asp36.
Background
Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (1-3). Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines (4,5). In addition, Snail blocks the cell cycle and confers resistance to cell death (6). Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function (7-10).
- Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-3161.
- Cano, A. et al. (2000) Nat. Cell Biol. 2, 76-83.
- Batlle, E. et al. (2000) Nat. Cell Biol. 2, 84-89.
- Yokoyama, K. et al. (2003) Int. J. Oncol. 22, 891-898.
- Yokoyama, K. et al. (2001) Oral Oncol. 37, 65-71.
- Vega, S. et al. (2004) Genes Dev. 18, 1131-1143.
- Zhou, B.P. et al. (2004) Nat. Cell Biol. 6, 931-940.
- Bachelder, R.E. et al. (2005) J. Cell Biol. 168, 29-33.
- Yook, J.I. et al. (2005) J. Biol. Chem. 280, 11740-11748.
- Yang, Z. et al. (2005) Cancer Res. 65, 3179-3184.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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