WB, IP
H M R Mk
Endogenous
80
Rabbit IgG
#Q9HAU4
64750
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro160 of human Smurf2 protein.
Background
Smad ubiquitin regulatory factor 2 (Smurf2) is a HECT domain E3 ubiquitin ligase. It was initially identified as an inhibitor of TGF-β/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (1-3). Subsequent studies have revealed its role in neuronal and planar cell polarity, as well as in the senescence response and suppression of tumorigenesis (4-8). Smurf2 has a broad range of substrates including RUNX2, AMSH, Rap1B, and RNF11 (5,9-11). Smurf2 is widely expressed in various tissues. The C2 domain of Smurf2 inhibits its catalytic activity by interacting with the HECT domain (12). Research studies have shown that Smurf2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (13).
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- Kavsak, P. et al. (2000) Mol Cell 6, 1365-75.
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- Zhang, H. and Cohen, S.N. (2004) Genes Dev 18, 3028-40.
- Schwamborn, J.C. et al. (2007) EMBO J 26, 1410-22.
- Narimatsu, M. et al. (2009) Cell 137, 295-307.
- Nie, J. et al. (2010) J Biol Chem 285, 22818-30.
- Ramkumar, C. et al. (2012) Cancer Res 72, 2714-9.
- Subramaniam, V. et al. (2003) Br J Cancer 89, 1538-44.
- Li, H. and Seth, A. (2004) Oncogene 23, 1801-8.
- Kaneki, H. et al. (2006) J Biol Chem 281, 4326-33.
- Wiesner, S. et al. (2007) Cell 130, 651-62.
- Blank, M. et al. (2012) Nat Med 18, 227-34.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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