WB, IP
H
Endogenous
120, 100
Rabbit IgG
#Q9HBG7
4063
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the intracellular domain of human SLAMF3/CD229 protein.
Background
Signaling lymphocytic activation molecule family 3 (SLAMF3/CD229) is a type-I transmembrane glycoprotein belonging to the SLAM family of immunoreceptors. Research studies have demonstrated that SLAMF3/CD229 is a homophilic, self-ligating receptor and expression is largely restricted to the surface of T cells and B cells (1,2). The extracellular domain of SLAMF3/CD229 contains a duplicated immunoglobulin (Ig)-like variable-type domain and a duplicated Ig-like C2-type domain, while the cytoplasmic domain is subject to alternative splicing and contains multiple potential tyrosine phosphorylation sites capable of recruiting signaling molecules such as EAT-2 and SAP (1,3,4). Research studies have shown that SLAMF3/CD229 is also highly overexpressed by multiple myeloma plasma cells and drives intracellular signaling cascades that promote plasma cell proliferation and survival (5-7).
- de la Fuente, M.A. et al. (2001) Blood 97, 3513-20.
- Romero, X. et al. (2005) J Immunol 174, 7033-42.
- Morra, M. et al. (2001) EMBO J 20, 5840-52.
- Sayós, J. et al. (2001) Blood 97, 3867-74.
- Ishibashi, M. et al. (2020) Mol Cancer Res 18, 632-643.
- Lin, Z. et al. (2022) Aging (Albany NY) 14, 9264-9279.
- Roncador, G. et al. (2022) Cancers (Basel) 14, 2154. doi: 10.3390/cancers14092154.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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