Revision 1

#12891Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IF-IC, FC-FP

REACTIVITY:

H M R Mk

SENSITIVITY:

Endogenous

MW (kDa):

36

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q15475

Entrez-Gene Id:

6495

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SIX1 (D4A8K) Rabbit mAb recognizes endogenous levels of total SIX1 protein. It does not cross-react with other SIX family proteins.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro249 of human SIX1 protein.

Background

Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in Drosophila mutant screens for embryonic eye development genes (1-3). The prototypical family member (sine oculis, so) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans (SIX1) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7).
In contrast to the Drosophila ortholog, the vertebrate SIX1 gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).

  1. Kumar, J.P. (2009) Cell Mol Life Sci 66, 565-83.
  2. Fischbach, K.F. and Technau, G. (1984) Dev Biol 104, 219-39.
  3. Fischbach, K.F. and Heisenberg, M. (1981) Proc Natl Acad Sci U S A 78, 1105-9.
  4. Boucher, C.A. et al. (1996) Genomics 33, 140-2.
  5. Pignoni, F. et al. (1997) Cell 91, 881-91.
  6. Ford, H.L. et al. (1998) Proc Natl Acad Sci U S A 95, 12608-13.
  7. Coletta, R.D. et al. (2004) Proc Natl Acad Sci U S A 101, 6478-83.
  8. Oliver, G. et al. (1995) Development 121, 4045-55.
  9. Kaiser, R. et al. (2007) Am J Med Genet A 143A, 2185-8.
  10. Fujimoto, Y. et al. (2013) Dev Cell 26, 416-30.
  11. Liu, Y. et al. (2013) PLoS One 8, e67762.
  12. Behbakht, K. et al. (2007) Cancer Res 67, 3036-42.
  13. Ng, K.T. et al. (2006) Br J Cancer 95, 1050-5.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
仅供研究使用。不得用于诊断流程。