WB, IP
M
Endogenous
32
Rabbit IgG
#Q8R216
75387
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala184 of mouse SirT4 protein.
Background
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, initially classified as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT4, a mammalian homolog of Sir2, is localized to the mitochondria and has been implicated in the regulation of cell metabolism, oxidative stress, genomic stability, and aging (2,3). In addition to deacetylase activity, multiple additional enzymatic activities have been attributed to SirT4, including protein deacylation, ADP-ribosylation, and NAD-dependent protein lipoamidase activity. SirT4 regulates the metabolism of glutamine, lipids, and glucose through direct deacetylation of malonyl CoA decarboxylase (MCD) (4), ADP-ribosylation of glutamine dehydrogenase (5), and hydrolysis of the lipoamide cofactors from the E2 component dihydrolipoyllysine acetyltransferase (DLAT) of the pyruvate dehydrogenase complex (PDHC) (6). SirT4 is a tumor suppressor protein, and expression is reduced or lost in a large number of cancers, resulting in the acceleration of tumor morbidity and metastasis (2,3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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