WB
H M R
Endogenous
19
Rabbit IgG
#Q5BJF2
27346
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro57 of human SIGMAR2/TMEM97 protein.
Background
Sigma intracellular receptor 2 (SIGMAR2 or TMEM97) is an endoplasmic reticulum (ER)-resident membrane chaperone protein that regulates the sterol transporter NPC1 (1). Recently, SIGMAR2/TMEM97 was identified as the opioid 2 receptor. Opioid receptors are a large super family of 7 transmembrane-spanning G protein-coupled receptors (GPCRs) that are activated by both endogenous opioid peptides and by exogenously administered opiate compounds (2). Opioid receptors are of pharmacological interest not only as analgesics but to treat neurological disorders including psychiatric and neurodegenerative diseases. Opioid receptors are classified pharmacologically, including σ1 and σ2 type receptors. Although SIGMAR1 was identified in 1996 as the σ1 receptor, identification of the σ2 type receptor remained elusive until 2017 when TMEM97 was identified as the σ2 type receptor (3,4). In addition to acting as an opioid receptor, SIGMAR2/TMEM97 may also act within a complex to regulate cellular update of Aβ, the pathological hallmark of Alzheimer's disease (5,6).
- Bartz, F. et al. (2009) Cell Metab 10, 63-75.
- Waldhoer, M. et al. (2004) Annu Rev Biochem 73, 953-90.
- Hanner, M. et al. (1996) Proc Natl Acad Sci U S A 93, 8072-7.
- Alon, A. et al. (2017) Proc Natl Acad Sci U S A 114, 7160-7165.
- Yang, K. et al. (2020) Molecules 25, 5439. doi: 10.3390/molecules25225439.
- Riad, A. et al. (2020) Mol Neurobiol 57, 3803-3813.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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