WB
Vir
Endogenous
9-14
Rabbit
#P0DTC7
43740573
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val74 of SARS-CoV-2 ORF7a protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SARS-CoV-2 ORF7a is a type-I transmembrane protein with an amino-terminal immunoglobulin (IgG)-like domain involved in immune modulation and a short cytosolic tail that contains an endoplasmic reticulum (ER) retention motif that mediates trafficking to the ER-Golgi intermediate complex (ERGIC) (1). Studies of ORF7a in SARS-CoV identified roles in apoptosis and cell cycle progression (2-4). Regulation of apoptosis has been attributed to an association with Bcl-xL, a key pro-survival Bcl-2 family member (5). Studies in both SARS-CoV and SARS-CoV-2 have shown that ORF7a can promote viral release through inhibition of BST2 (6,7). There are also reports that SARS-CoV-2 contributes to immune regulation as part of the viral life cycle. It can inhibit type I interferon signaling through inhibition of STAT2 phosphorylation (8). However, it can also lead to activation of NF-κB and induce the expression of inflammatory cytokines (9). ORF7a has also been shown to inhibit late stages of autophagy by reducing lysosomal acidity (10).
- Redondo, N. et al. (2021) Front Immunol 12, 708264.
- Schaecher, S.R. et al. (2007) J Virol 81, 11054-68.
- Tan, Y.J. et al. (2004) J Virol 78, 14043-7.
- Yuan, X. et al. (2006) Virology 346, 74-85.
- Tan, Y.X. et al. (2007) J Virol 81, 6346-55.
- Taylor, J.K. et al. (2015) J Virol 89, 11820-33.
- Martin-Sancho, L. et al. (2021) Mol Cell 81, 2656-2668.e8.
- Xia, H. et al. (2020) Cell Rep 33, 108234.
- Su, C.M. et al. (2021) Sci Rep 11, 13464.
- Koepke, L. et al. (2021) Autophagy 17, 2659-2661.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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