WB
H M R Mk
Endogenous
21
Rabbit
#P62745
388
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RhoB. Antibodies are purified using protein A and peptide affinity chromatography.
Background
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).
RhoB functions in the regulation of cell shape, migration and adhesion (4). RhoB activity has also been shown to play a role in protein trafficking (5,6) and in CXCR2-mediated chemotaxis (6). Inhibition of RhoB activity downstream of PKCι influences the degree of invasion and migration by glioblastoma cells (7), and RhoB expression has a negative affect on tumor growth in ovarian cancer (8).
- DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
- Wheeler, A.P. and Ridley, A.J. (2007) Exp Cell Res 313, 3505-16.
- Sandilands, E. et al. (2007) J Cell Sci 120, 2555-64.
- Neel, N.F. et al. (2007) J Cell Sci 120, 1559-71.
- Baldwin, R.M. et al. (2008) Oncogene 27, 3587-95.
- Couderc, B. et al. (2008) Cancer Gene Ther 15, 456-64.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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