WB, IF-IC
H
Endogenous
90
Rabbit IgG
#Q96RL1
51720
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly321 of human RAP80 protein.
Background
The breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 protein function. The BRCA1-RAP80 complex (BRCA1 A complex) includes RAP80, BRCC36, BRE, Abraxas, and NBA1 and functions in G2/M phase checkpoint control (reviewed in 1,2).
The ubiquitously expressed receptor-associated protein 80 (RAP80, UIMC1) is required for recruitment and stability of the BRCA1 A complex at sites of DNA damage (3). Research studies indicate that the absence of RAP80 in cells results in increased sensitivity to the topoisomerase II inhibitor etoposide (4). In the absence of functional RAP80, BRCA1 A complex function is suppressed and cells become more sensitive to DNA damage-induced genome instability (5,6). Phosphorylation of RAP80 by CDK1/Cyclin B at Ser177 regulates RAP80 function at the mitotic checkpoint (7). A naturally occurring in-frame deletion mutant within RAP80 likely alters RAP80 protein-protein interactions and is associated with an increase in chromosomal abnormalities (8,9).
- Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
- Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
- Wu, J. et al. (2012) J Biol Chem 287, 22919-26.
- Iijima, J. et al. (2010) Cancer Res 70, 8467-74.
- Bian, C. et al. (2012) PLoS One 7, e40406.
- Yin, Z. et al. (2012) Cancer Res 72, 5080-90.
- Cho, H.J. et al. (2013) J Biol Chem 288, 3768-76.
- Nikkilä, J. et al. (2009) Oncogene 28, 1843-52.
- Anamika et al. (2014) J Biol Chem 289, 12852-62.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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