WB
H
Endogenous
240
Rabbit IgG
#Q12913
5795
Product Information
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp440 of human PTPRJ/DEP-1 protein.
Background
Protein-tyrosine phosphatase receptor type J/density-enhanced phosphatase-1 (PTPRJ/DEP-1) is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ/DEP-1 functions as a tumor suppressor and its expression is lost in multiple types of human cancers (7,8).
- Honda, H. et al. (1994) Blood 84, 4186-94.
- de la Fuente-García, M.A. et al. (1998) Blood 91, 2800-9.
- Tangye, S.G. et al. (1998) J Immunol 161, 3249-55.
- Ostman, A. et al. (1994) Proc Natl Acad Sci U S A 91, 9680-4.
- Palka, H.L. et al. (2003) J Biol Chem 278, 5728-35.
- Kovalenko, M. et al. (2000) J Biol Chem 275, 16219-26.
- Venkatachalam, R. et al. (2010) Gastroenterology 139, 2221-4.
- Aya-Bonilla, C. et al. (2013) Genes Chromosomes Cancer 52, 467-79.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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