| Kit Includes | Quantity | Antigen Retrieval / Diluent | Isotype |
|---|---|---|---|
| Cleaved Caspase-3 (Asp175) Antibody #9661 | 40 µl | Citrate / SignalStain® Antibody Diluent #8112 | |
| Phospho-Histone H3 (Ser10) Antibody #9701 | 40 µl | Citrate / SignalStain® Antibody Diluent #8112 | |
| PCNA (PC10) Mouse mAb #2586 | 40 µl | Citrate / SignalStain® Antibody Diluent #8112 | Mouse IgG2a |
| Survivin (71G4B7) Rabbit mAb #2808 | 40 µl | Citrate / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| *SignalStain® Antibody Diluent #8112 | 25 ml | ||
| †SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104 | 1 Pack |
*SignalStain® Antibody Diluent is supplied as a working solution and should be stored at 4ºC (packaged separately).
†Control slides should be stored at 4ºC (packaged separately).
IHC-P
#P68431, #P12004, #P42574, #O15392
8350, 5111, 836, 332
Product Information
Storage
Specificity / Sensitivity
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein, or with a synthetic peptide corresponding to residues surrounding Cys60 of human Survivin. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3, or with a synthetic peptide surrounding amino-terminal residues adjacent to Asp175 of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.
Product Description
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments (2). Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). PCNA protein expression is a well-accepted marker of proliferation, and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. Histone H3, H2A, H2B, and H4 make up the core histones of the nucleosome. In response to various stimuli, the amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination, which affect the accessibility of chromatin to transcription factors (8). Phosphorylation of Histone H3 at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (9-11).
- Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
- Nicholson, D.W. et al. (1995) Nature 376, 37-43.
- Reed, J.C. and Reed, S.I. (1999) Nat Cell Biol 1, E199-200.
- Li, F. et al. (1998) Nature 396, 580-4.
- Li, F. et al. (1999) Nat Cell Biol 1, 461-6.
- Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20.
- Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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