WB, IP
H
Endogenous
170
Rabbit IgG
#Q9HAZ2
63976
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro666 of human PRDM16 protein.
Background
PRDM16 is a transcription factor that is structurally characterized by a conserved N-terminal PRDI-BF1 and RIZ1 homology (PR) domain and a variable number of zinc fingers (1,2). The PR domain bears similarities to the catalytic suppressor of variegation 3–9, enhancer of zeste, and trithorax (SET) domain that is found in a group of histone methyltransferases (1,3). Indeed, PRDM16 has been shown to catalyze mono-methylation of histone 3 at lysine (H3K9me1) (4). Human PRDM16 encodes a protein with four isoforms with the most extensively studied isoforms being the full-length PRDM16 and the PR-lacking isoform that is generated by a transcript derived from an internal promoter (5). PRDM16 is involved in the regulation of various biological processes, including adipose biology and energy homeostasis. This protein is also expressed in hematopoietic stem cells (HSCs) and plays a key role in the establishment and maintenance of these cells (6,7). More recently, PRDM16 was shown to be a suppressor of lung adenocarcinoma metastasis, and its low expression predicted poor prognosis of lung adenocarcinoma patients (8).
- Hohenauer, T. and Moore, A.W. (2012) Development 139, 2267-82.
- Fog, C.K. et al. (2012) Bioessays 34, 50-60.
- Di Zazzo, E. et al. (2013) Biology (Basel) 2, 107-41.
- Pinheiro, I. et al. (2012) Cell 150, 948-60.
- Nishikata, I. et al. (2003) Blood 102, 3323-32.
- Yoshida, M. et al. (2004) Blood 103, 2753-60.
- Yu, H. et al. (2014) Blood 124, 1737-47.
- Fei, L.R. et al. (2019) J Exp Clin Cancer Res 38, 35.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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