WB, IF-F
H M R
Endogenous
20-30
Rabbit IgG
#P60201
5354
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #36593.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PLP1 protein.
Background
Myelin proteolipid protein (PLP1) corresponds to the majority of myelin proteins in the CNS, providing support to axons and modulating the axonal growth (1). DM20 is the result of the alternative splicing of the plp1 gene, which is linked to oligodendrocyte differentiation and survival. The imbalance of PLP1/DM20 is linked to Pelizaeus-Merzbacher disease (2,3), including mitochondrial damage (4). In addition, PLP1, but not DM20, can enter the mitochondria and participate in the metabolism of cells (5).
- Thomson, C.E. et al. Dev Neurosci 27, 27-36.
- Laššuthová, P. et al. (2014) J Child Neurol 29, 924-31.
- Dhaunchak, A.S. et al. (2011) J Neurosci 31, 14961-71.
- Ruiz, M. et al. (2017) Brain Pathol 28, 611-630. doi: 10.1111/bpa.12571. Epub 2017 Dec 26.
- Somayajulu, M. et al. (2018) Neurosci Lett 678, 90-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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