WB
H M R
Endogenous
28, 29
Rabbit
#Q92530
9491
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro245 of human PI31/PSMF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Proteasome-binding protein PI31 (Proteasomal Inhibitor of 31kD) is encoded by the PSMF1 gene and regulates proteasome function in the cell, which is essential for cell function and survival. Regulated degradation of intracellular proteins is mediated by the ubiquitin-proteasome system (UPS) by a multi-step process (1,2). First, protein to be degraded is tagged with a chain of ubiquitin molecules. Ubiquitinated proteins are then cleaved into small peptides by the 26S proteasome. Proteasome activity by the UPS is regulated at additional levels, including transcriptional expression of proteasomal proteins, posttranslational modification, and by various regulatory proteins. PI31/PSMF1 is a structurally conserved proteasome regulator that binds to the 20S proteasome and functions to inhibit 20S proteasome hydrolysis of small peptides (3,4). Unexpectedly, PI31/PSMF1 promotes UPS-mediated protein breakdown in vivo, suggesting additional proteasome regulatory functions beyond inhibition of the 20S proteasome (5). PI31/PSMF1 also serves as an adaptor protein to transport proteasomes via cellular protein motors (6,7). This process may be particularly important in neurons where local proteasomal degradation at distal tips of neurons may play a critical role in the maintenance of neuronal architecture, circuitry, and function.
- Collins, G.A. and Goldberg, A.L. (2017) Cell 169, 792-806.
- Pohl, C. and Dikic, I. (2019) Science 366, 818-822.
- Chu-Ping, M. et al. (1992) Biochim Biophys Acta 1119, 303-11.
- McCutchen-Maloney, S.L. et al. (2000) J Biol Chem 275, 18557-65.
- Bader, M. et al. (2011) Cell 145, 371-82.
- Minis, A. et al. (2019) Proc Natl Acad Sci U S A 116, 24639-24650.
- Liu, K. et al. (2019) Dev Cell 50, 509-524.e10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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