Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb (#3004) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, E-P

REACTIVITY:

All

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Peptide ELISA (DELFIA)	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb detects endogenous levels of proteins containing phospho-threonine followed by proline and glutamine residues. This antibody also recognizes peptides and proteins containing the phospho-Thr-Pro motif.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-Thr-Pro-Glu motif.

Background

The phospho-Thr-Pro-Glu motif is a subgroup of the phospho-Thr-Pro motif. The Thr-Pro motif is phosphorylated by proline-directed protein kinases, such as MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in many cellular processes, such as cell proliferation and differentiation (1-6). This phospho-Thr-Pro-Glu antibody provides a useful tool to study a subset of substrates of proline-directed kinases.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation E-P: Peptide ELISA (DELFIA)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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