| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Tau (D1M9X) XP® Rabbit mAb | 46687 | 20 µl | 50-80 kDa | Rabbit IgG |
| Phospho-Tau (Ser202) (D4H7E) Rabbit mAb | 39357 | 20 µl | 50-80 kDa | Rabbit IgG |
| Phospho-Tau (Ser396) (PHF13) Mouse mAb | 9632 | 20 µl | 50-80 kDa | Mouse IgG2b |
| Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb | 20194 | 20 µl | 50-80 kDa | Rabbit IgG |
| Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb | 35834 | 20 µl | 50-80 kDa | Rabbit IgG |
| Phospho-Tau (Ser416) (D7U2P) Rabbit mAb | 15013 | 20 µl | 50-80 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Phospho-Tau Family Antibody Sampler Kit provides an economical means of detecting the activation of Tau family members using phospho-specific and control antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Storage
Background
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
Investigators have shown that Tau phosphorylation at Ser404 destabilizes microtubules and that Tau is hyperphosphorylated at Ser404 in Alzheimer's disease (2,4-6). Research studies indicate that calcium-/calmodulin-dependent protease kinase II (CAM-kkinase II) is responsible for the phosphorylation of Tau at Ser416. Phosphorylated Tau protein is localized with neuronal soma or hippocampal neurons and immortalized GnRH neurons (7). Investigators have shown that Tau is phosphorylated during development and hyperphosphorylated at Ser202 in Alzheimer's disease (8).
- Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
- Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
- Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
- Shiurba, R.A. et al. (1996) Brain Res 737, 119-32.
- Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83.
- Bertrand, J. et al. (2010) Neuroscience 168, 323-34.
- Yamamoto, H. et al. (2005) J Neurochem 94, 1438-47.
- Goedert, M. et al. (1993) Proc Natl Acad Sci U S A 90, 5066-70.
Background References
Trademarks and Patents
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