WB, IF-IC
H
Endogenous
160
Rabbit
#Q9Y6Q9
8202
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequence surrounding Thr24 of the human SRC-3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).
Phosphorylation of Thr24 of SRC-3 proteins can be induced by stimulation with EGF. Phosphorylated SRC-3 translocates from the cytoplasm to the nucleus where it interacts with other transcription factors and steroid hormone receptors and regulates gene expression (15).
- Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
- Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
- Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
- Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
- Spencer, T.E. et al. (1997) Nature 389, 194-198.
- Chen, H. et al. (1997) Cell 90, 569-580.
- Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
- Chen, D. et al. (1999) Science 284, 2174-2177.
- Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
- Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
- Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
- Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
- Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
- Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.
- Amazit, L. et al. (2007) Mol. Cell Biol. 27, 6913-6932.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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