WB
H Mk
Endogenous
45
Rabbit
#P18754
1104
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser11 of human RCC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 (RCC1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity (6).
- Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
- Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
- Moore, W. et al. (2002) Curr Biol 12, 1442-7.
- Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
- Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
- Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
- Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
- Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
- Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
- Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
- Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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