Phospho-Rad17 (Ser645) Antibody #3421
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Inquiry Info. # 3421
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Supporting Data
| REACTIVITY | |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Product Information
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-Rad17 (Ser645) Antibody detects endogenous levels of hRad17 only when phosphorylated at serine 645. The antibody does not cross-react with Rad17 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser645 of human Rad17. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).
- Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
- Li, L. et al. (1999) Oncogene 18, 1689-99.
- Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
- von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
- Bao, S. et al. (2001) Nature 411, 969-74.
- Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
- Wang, X. et al. (2001) Cancer Res 61, 7417-21.
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