| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb | 4370 | 80 µl | 44, 42 kDa | Rabbit IgG |
| Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb | 4668 | 40 µl | 46, 54 kDa | Rabbit IgG |
| Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb | 4631 | 40 µl | 43 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 40 µl | Goat | |
| U0126 | 9903 | 0.3 mg |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Phospho-MAPK Family Rabbit mAb Sampler Kit contains 40 µl of each primary rabbit monoclonal antibody [Phospho-p44/42 MAPK (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-p38 MAPK (Thr180/Tyr182)], 50 µl of anti-rabbit IgG secondary antibody (HRP conjugated) and 0.3 mg of the MEK1/2 Inhibitor (U0126).
Storage
Background
p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).
- Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
- Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
- Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
- Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.
Background References
Trademarks and Patents
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