WB
H M R
Endogenous
98
Rabbit
#Q9UQC2
9846
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 of human Gab2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).
Akt phosphorylates Gab2 at Ser159. This phosphorylation inhibits Gab2 tyrosine phosphorylation and downstream signal amplification (10).
- Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
- Weidner, K.M. et al. (1996) Nature 384, 173-176.
- Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
- Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
- Lehr, S. et al. (1999) Biochemistry 38, 151-159.
- Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
- Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
- Gual, P. et al. (2000) Oncogene 19, 1509-18.
- Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.
- Lynch, D.K. and Daly, R.J. (2002) EMBO J 21, 72-82.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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