WB, W-S, IP
H
Endogenous
29
Rabbit IgG
#Q9UN19
27071
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr139 of human DAPP1/BAM32 protein.
Background
The dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1/BAM32) is a cytoplasmic adaptor protein that mediates the recruitment and interaction of molecules required for signal transduction downstream of the B cell receptor (BCR) (1). The DAPP1/BAM32 protein contains an amino-terminal SH2 domain and a carboxy-terminal pleckstrin homology (PH) domain that binds to PI3K-derived phosphoinositides (i.e., PIP3). Upon BCR activation, DAPP1/BAM32 is phosphorylated at specific tyrosine residues and translocated from the cytoplasm to the membrane. Research studies indicate that phosphorylation and translocation of DAPP1/BAM32 is strongly dependent upon PI3K signaling (2,3). The amino-terminal SH2 domain binds to PLCγ2 and other tyrosine-phosphorylated targets. As a result of these interactions, DAPP1/BAM32 can adjust the response to receptor activation by coordinating membrane-localized interactions among proteins of distinct signal transduction pathways (1,4). DAPP1/BAM32 is expressed most abundantly in B lymphocytes; high expression during dendritic cell (DC) maturation and localization to contact sites between DC and allogenic T cells suggest that the DAPP1/BAM32 adaptor may play a role in the activation of T cells through MHC class I-mediated signaling pathways (5).
Research studies show that phosphorylation of DAPP1/BAM32 at Tyr139 is PI3K-dependent, requires an intact PH domain in DAPP1/BAM32, and is likely performed by Src-family kinases following membrane recruitment of DAPP1/BAM32 by phosphoinositides (6). Blocking phosphorylation of DAPP1/BAM32 at Tyr139 inhibits BCR internalization and reduces cellular F-actin levels, suggesting that phosphorylation of DAPP1/BAM32 may play a role in regulating actin-dependent internalization of the activated BCR (7,8).
- Marshall, A.J. et al. (2007) Biochem Soc Trans 35, 181-2.
- Marshall, A.J. et al. (2000) J Exp Med 191, 1319-32.
- Anderson, K.E. et al. (2000) Curr Biol 10, 1403-12.
- Richards, S. et al. (2008) Immunol Rev 224, 183-200.
- Ortner, D. et al. (2011) J Immunol 187, 3972-8.
- Dowler, S. et al. (2000) Biochem J 349, 605-10.
- Niiro, H. et al. (2004) J Immunol 173, 5601-9.
- Allam, A. et al. (2004) J Biol Chem 279, 39775-82.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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