| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-Bad (Ser112) (40A9) Rabbit mAb | 5284 | 20 µl | 23 kDa | Rabbit IgG |
| Phospho-Bad (Ser136) (D25H8) Rabbit mAb | 4366 | 20 µl | 23 kDa | Rabbit IgG |
| Phospho-Bad (Ser155) Antibody | 9297 | 20 µl | 23 kDa | Rabbit |
| Bad (D24A9) Rabbit mAb | 9239 | 20 µl | 23 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Phospho-Bad Antibody Sampler Kit provides an economical means to investigate the role of Bad protein in apoptosis. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.
Storage
Background
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
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- Harada, H. et al. (1999) Mol. Cell 3, 413-422.
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- Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
- Datta, S. et al. (2000) Mol. Cell 6, 41-51.
Background References
Trademarks and Patents
限制使用
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