Revision 3

#9566

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
92

Source/Isotype:
Rabbit

UniProt ID:
#P35222

Entrez-Gene Id:
1499

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Phospho-β-Catenin (Ser552) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser552.

Species predicted to react based on 100% sequence homology

Rat, Chicken, Xenopus, Zebrafish

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin. Antibodies are purified by peptide affinity chromatography.

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Both Akt and PKA were shown to phosphorylate β-catenin at Ser552. Phosphorylation at Ser552 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

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Western blot analysis of total cell lysates from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes, or the lysate was treated with λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser552) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (bottom).
Western Blotting Image 1: Phospho-β-Catenin (Ser552) Antibody
COS-7 cells were transfected with cDNAs for the DYKDDDDK-tagged wild type (WT) β-catenin or for Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting with Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody #9567, or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine / Pulmonary, The University of Chicago).
Western Blotting Image 2: Phospho-β-Catenin (Ser552) Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.