WB, IP, IHC-P
H
Endogenous
90
Rabbit IgG
#Q15063
10631
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #79950.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser395 of human periostin protein.
Background
Periostin (POSTN, OSF2) is a secretory matricellular protein that belongs to the fasciclin family. The protein has multiple functional domains that interact with different extracellular matrix (ECM) components (1-2). The N-terminal EMI domain of periostin interacts with fibronectin as well as collagens and lysyl oxidase. The middle part of the protein consists of four FAS 1 (fasciclin) domains which are responsible for the protein association with tenascin-C and CCN3. The C-terminal CTD domain assumes an inhibitory effect on FAS1 domain function. Lastly, the C-terminal HBD domain mediates proteoglycan association (1-2). Periostin has been shown to mediate important cellular processes such as ECM stemness, mechanical stress response, collagen cross-linking and fibrogenesis, cell proliferation, survival, and adhesion (1-3). It is an important modulator in multiple disease developments and has been frequently used as a biomarker for cancer, fibrosis, inflammation, and tissue wound healing (4-7).
- Kudo, A. (2017) Cell Mol Life Sci 74, 4259-68.
- Kudo, A. and Kii, I. (2018) J Cell Commun Signal 12, 301-8.
- Conway, S.J. et al. (2014) Cell Mol Life Sci 71, 1279-88.
- González-González, L. and Alonso, J. (2018) Front Oncol 8, 225.
- O'Dwyer, D.N. and Moore, B.B. (2017) Cell Mol Life Sci 74, 4305-14.
- Li, W. et al. (2015) Respir Res 16, 57.
- Walker, J.T. et al. (2016) Cell Tissue Res 365, 453-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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