| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody | 2606 | 20 µl | 61 to 67 (PAK2), 68 to 74 (PAK1/3) kDa | Rabbit |
| Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody | 2605 | 20 µl | 61 to 67 (PAK2), 68 to 74 (PAK1/3) kDa | Rabbit |
| Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody | 2601 | 20 µl | 61 to 67 (PAK2), 68 to 74 (PAK1/3) kDa | Rabbit |
| Phospho-PAK2 (Ser20) Antibody | 2607 | 20 µl | 61 to 67 kDa | Rabbit |
| PAK1 Antibody | 2602 | 20 µl | 68 kDa | Rabbit |
| PAK2 (C17A10) Rabbit mAb | 2615 | 20 µl | 61 kDa | Rabbit IgG |
| PAK3 Antibody | 2609 | 20 µl | 65 kDa | Rabbit |
| PAK1/2/3 Antibody | 2604 | 20 µl | 61 (PAK2), 68 (PAK1/3) kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The PAK antibody sampler kit provides and economical means to evaluate the activation status of PAK1, 2, and 3. This kit includes enough primary and secondary antibodies to perform two western blots with each antibody.
Storage
Background
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation of Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8).
- Knaus, U.G. and Bokoch, G.M. (1998) Int J Biochem Cell Biol 30, 857-62.
- Daniels, R.H. et al. (1998) EMBO J 17, 754-64.
- King, C.C. et al. (2000) J Biol Chem 275, 41201-9.
- Manser, E. et al. (1997) Mol Cell Biol 17, 1129-43.
- Gatti, A. et al. (1999) J Biol Chem 274, 8022-8.
- Lei, M. et al. (2000) Cell 102, 387-97.
- Chong, C. et al. (2001) J Biol Chem 276, 17347-53.
- Zhao, Z.S. et al. (2000) Mol Cell Biol 20, 3906-17.
Background References
Trademarks and Patents
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